Efficient Operation Guidelines for Fermentation Tanks

 
The rapid advancement in the field of biotechnology necessitates reliable fermentation equipment to support various applications such as biopharmaceuticals, food processing, and biofuel production. Among the core equipment, fermentation tanks play a pivotal role, with the accuracy and stability of their mechanical operations directly impacting production efficiency and product quality. Therefore, proper operation of fermentation tanks is crucial to ensure the smooth progress of biotechnological processes. This guide provides detailed operational steps to ensure the safety, efficiency, and reliability of mechanical operations in fermentation tanks.
 

1. Preparation Phase

 
Before commencing operations, comprehensive preparation of the fermentation tank is essential. Ensure all necessary electrodes are correctly installed and connected to their respective control systems. Prior to sterilization, meticulous inspection of all interfaces and pipelines of the fermentation tank is necessary to ensure there are no abnormalities, with particular attention to the integrity of steam pipelines. Simultaneously, check the operation of the internal stirrer to ensure its normal functioning. Before initiating operations, ensure all valves are fully closed and are inspected by at least two operators to ensure safety.
 

2. Sterilization Process

 
Sterilization is a crucial step in the fermentation process as it effectively eliminates microbes that could affect fermentation. This process requires two operators, one to operate various valves, and the other to observe and record parameters. Firstly, confirm all valves are closed, then open the water inlet valve of the steam generator and start the steam generator. Subsequently, open the drain valve to eliminate rust and monitor the water level to prevent accidents. Gradually open various valves to introduce steam into the jacket of the fermentation tank until the required temperature and pressure for sterilization are reached. Throughout the sterilization process, closely monitor changes in steam pressure and adjust valves accordingly to maintain stable pressure. Once the sterilization temperature is reached, start the timer.
 

3. Fermentation Phase

 
Fermentation is the core of the entire process, where mechanical operations are particularly crucial. By controlling the speed of the internal stirrer in the fermentation tank, adequate mixing of nutrients in the medium is ensured, providing an optimal growth environment for microorganisms. Simultaneously, adjusting the airflow and temperature of the fermentation tank meets the oxygen requirements and growth temperature of microorganisms. During fermentation, closely monitor parameters such as temperature, pressure, and pH within the fermentation tank to ensure the process is in optimal condition. If any abnormalities occur, such as poor bubble generation or high temperature, take prompt measures to adjust.
 

4. Precautions

 
Safety should always be a priority during operations. Wearing long-sleeved clothing, long pants, and protective gloves is necessary to prevent burns. If the steam generator automatically fills with water, leading to a drop in steam pressure, carefully observe and adjust valves promptly to maintain stable pressure. In the later stages of fermentation, air vent blockages may occur, which can be alleviated by rotating the air release valve. Additionally, monitor the pressure of air filters to ensure they do not exceed specified limits and consider the impact of condensation on liquid concentration within the fermentation tank.
 
By strictly adhering to the mechanical operation steps provided in this guide, you can ensure the smooth operation of fermentation tanks in biotechnology. This not only enhances production efficiency and reduces production costs but also safeguards product quality, contributing to the advancement of the biotechnology field.